lowest common ancestor in the tree for an average offline
Basic data structure trong lập trình
Ngày tải lên :
11/05/2015, 05:53
... vertices in it, in the other - the depth of the tree (or rather, the upper limit to the depth of the tree, as the joint application of heuristics compression ways the real depth of the tree can ... (lowest common ancestor in the tree) for an average Offline Tarjan algorithm for finding the LCA for an average online is described in the relevant article This algorithm compares favorably with other ... and then go to these lists in order, each time passing in the following list using predposchitannyh pointers, and taking one step to the left, thereby taking into account that half the following...
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Tài liệu Powerful Offline Marketing In The Internet Age ppt
Ngày tải lên :
15/12/2013, 10:15
... for marketing offline, which is something more online Marketers should be doing Ideally we should all be marketing both offline and online Well these folks delivered the goods, and while the call ... go about bringing in this “found” business, there are experienced marketers out there who could handle the nuts and bolts of the campaign In other words, this would be the reverse of the previous ... and my original Silent Tempest painting has been on display in the Wadsworth Atheneum In Hartford since 1998 That means if you hold onto your painting, you’ll likely see its value increase considerably...
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Tài liệu Murray N. Rothbard - History Of Money And Banking In The United States (pdf) pptx
Ngày tải lên :
17/01/2014, 02:20
... developing initial hypotheses and testing them in the light of available evidence The initial hypotheses come from the body of economic theory that has evolved in the past 200 years and is being ... logically inferring from them the laws governing the valuing and pricing of the means or “goods.” As such, economics does not inquire into the individual’s motivations in valuing and choosing specific ... identifies all the causal factors involved, the likelihood of the forecast event being realized depends on the actor having solved the weighting problem The uncertainty inherent in forecasting, therefore,...
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Tài liệu Engineering Mechanics - StaticsChapter 1Problem 1-1 Represent each of the following combinations of units in the correct SI form using an appropriate prefix: (a) m/ms (b) μkm (c) ks/mg (d) km⋅ μN Units Used: μN = 10−6N kmμkm = 109−6Gs = 10 s pptx
Ngày tải lên :
17/02/2014, 14:20
... Problem 2-18 If the tension in the cable is F1, determine the magnitude and direction of the resultant force acting on the pulley This angle defines the same angle θ of line AB on the tailboard ... determine the orientation θ of the third chain,measured clockwise from the positive x axis, so that the magnitude of force F in this chain is a minimum All forces lie in the x-y plane.What is the ... reproduced, in any form or by any means, without permission in writing from the publisher Engineering Mechanics - Statics Chapter Problem 2-27 The beam is to be hoisted using two chains Determine the...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... Protein targeting to the microsomes requires the cotranslational insertion of the newly synthesized protein into the microsomal membrane, where the N-terminal hydrophobic signal sequence of the ... located inside the innermembrane compartment In the third approach, we used alkaline Na2CO3 extraction to determine whether mitochondrial CYP2E1 is a membrane-intrinsic or membrane-extrinsic protein ... Role of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches The first approach involved measuring the level of...
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Tài liệu Báo cáo khoa học: High affinity copper binding by stefin B (cystatin B) and its role in the inhibition of amyloid fibrillation docx
Ngày tải lên :
19/02/2014, 06:20
... histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although there are four histidines in the C-terminal, another ... (Fig 2) The binding isotherm data were fitted to sequential binding site parameters and the best fit, producing the smallest v2 values, indicated two binding sites, both with affinities in the picomolar ... Copper binds to cystatin B change but lacks any copper binding capacity This point mutation lies within the C-terminal region and therefore could affect the structure of this part of the protein However,...
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Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc
Ngày tải lên :
19/02/2014, 07:20
... protein with an N-terminal extension reading MSPIHHHHHHLVPRGSV The first amino acid of mao is valine (underlined V in the one letter amino acid code) The protein eluted from the nickel-chelating ... other members of the polyamine oxidases, the FAD cofactor was noncovalently bound to the apoprotein and the C-terminal fingerprint sequence SGGCY of monoamine oxidases, with C being the cysteine ... complete mineralization of the pyrrolidine ring of nicotine, which after oxidation by 6-hydroxy-l-nicotine oxidase, is cleaved off from the pyridine ring of nicotine in the form of CH3-4-aminobutyrate...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
Ngày tải lên :
19/02/2014, 13:20
... lysine reduces the probability of forming the fully closed form of the protein The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine 120 in the ... provided further insight into the molecular mechanism driving the formation of the covalent C115–fosfomycin adduct A conservative exchange of K22 to arginine maintains fosfomycin binding, while ... mutant proteins This was interpreted as a lack of fosfomycin binding to these mutant proteins [12] Binding of fosfomycin to either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG...
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Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
Ngày tải lên :
19/02/2014, 17:20
... 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal structures of the N-glycans of GAT1, N-glycosylation processing of NNN was inhibited ... blotting Either antiGFP mAb MAB2510 or anti-GAT1 pAb was used for the immunostaining The protein bands obtained in western blotting were analyzed by phosphoimager scanning The total protein of the ... were used for measurement of the remaining [3H]GABA and [14C]sucrose The protein concentration in the supernatant was determined using the bicinchoninic acid protein assay reagent (Pierce, USA)...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
Ngày tải lên :
20/02/2014, 11:20
... impairment in the inner membrane The decrease in enzymatic Lon protease activity may be the result of either a loss of efficiency in relation to damaged protein and/or a decrease in the amount ... this protein in the matrix We have not detected any significant modification in the amount of this protein in the matrix We can conclude that the decrease in this enzymatic activity is the result ... that there is intracellular lipid glycoxidation that affects the phosphatidylethanolamine from membranes of these organelles [11] In the present investigation, we detected CML proteins using the...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
Ngày tải lên :
07/03/2014, 05:20
... unfolding of some protein substrates is initiated through productive binding to the substrate via two elements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer In the ... controls binding of ClpA to ClpP [14] Interestingly, there is also considerable debate regarding the role of the ClpB N-domain (which shares a common fold with the N-domain of ClpA) in substrate ... N-domain and two ATP-binding domains referred to as the D1 and D2 domains Interestingly, deletion of the N-domain from ClpA not only abolishes binding of the adaptor protein, ClpS, but additionally...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
Ngày tải lên :
07/03/2014, 17:20
... bound to any membrane used as the signal hardly changes during the injection and was the same as before the injection during the dissociation phases Weak binding at the micromolar range was observed ... surface-seeking potential Insertion into lipid monolayers was next measured using monolayers composed of DOPC or DOPG The insertion of proteins into the monolayer generated an increase in surface ... By following the kinetics of fibril formation, conditions were defined in which the protein exists in the form of prefibrillar oligomers ⁄ aggregates, which persist during the lag phase These have...
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Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx
Ngày tải lên :
07/03/2014, 21:20
... agonist binding but not antagonist binding [14] The corresponding residue in the OTR is also an arginyl (Arg34) and furthermore this arginyl is required for high affinity agonist binding to the OTR ... off-state The unique ability of arginyl to fulfil this constraining function in the N-terminus of the V1aR is similar to the unique constraining role of alanyl previously reported for Ala293 in the ... as the tracer ligand Binding data were analyzed by nonlinear regression to fit theoretical Langmuir binding isotherms to the experimental data using the FIG P program (Biosoft, Cambridge, UK) Individual...
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Báo cáo khoa học: Functional symmetry in the isolated domain demonstrated by N-ethylmaleimide labelling pdf
Ngày tải lên :
08/03/2014, 08:20
... chain to the nucleotide-binding pocket [3] We describe the engineering, expression and purification of single-cysteine-containing proteins comprising either the C-terminal NBD or the N-terminal ... N-terminal NBD of P-glycoprotein contains only a single cysteine in the Walker-A motif [43] Thus, MBP-NBD-Nter is a 75-kDa fusion protein containing a single cysteine residue located in the ATP-binding ... on p[NH]ppA binding, in agreement with the results obtained here The nonidentical interaction of GTP may be related to another variation in sequence in the vicinity of the ATP-binding pocket,...
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Báo cáo khoa học: Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) ppt
Ngày tải lên :
08/03/2014, 10:20
... University) [22,23], containing a cDNA encoding the insulin signal sequence and the Protein A-IgG binding domain, was inserted into NheI-HindIII digested pcDNA3.1, producing the vector, pInsProA pDN42 was ... GalNAc-T1, might be involved in the catalytic function of the enzyme To investigate whether the cysteine residues modified by PCMPS are involved in the binding of UDP-GalNAc, we treated recombinant GalNAc-T1 ... deleted from the rat isozyme, and an insulin signal sequence and a Protein A-IgG binding domain were fused to the resulting N terminus of the sequence The recombinant truncated GalNAc-T1 was then expressed...
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Báo cáo khoa học: Characterization of N-glycosylation consensus sequences in the Kv3.1 channel pot
Ngày tải lên :
16/03/2014, 13:20
... Adjustments of the cell fractionation protocol involved reducing the starting material and the volume of the sucrose layers but maintaining the relative ratios of the sucrose layers Infected Sf9 ... Endo H, the lowest band becomes the predominant form in all three instances The band observed for N220Q ⁄ N229Q does not shift in the presence of Endo H These results indicate that the upper band ... independently or together, the immunobands corresponded to the two faint bands of wild-type Kv3.1 or the lowest faint band, respectively These results indicate that the most prominent band of wild-type...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
Ngày tải lên :
16/03/2014, 18:20
... domain ERM contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually specific to ERM, and the C-terminal ... complexes (B) The binding of in vitro translated ERM is eliminated by mutations in Ets motifs The binding of in vitro translated ERM over the PS1 promoter was assayed by EMSAs including the 32P-labeled ... flanking the +1 site (A) The binding of nuclear factors is eliminated by mutations in Ets motifs The binding of nuclear factors present in SK-N-SH (K) or SH-SY5Y (H) nuclear extracts over the...
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Báo cáo khoa học: Role of the hinge peptide and the intersubunit interface in the swapping of N-termini in dimeric bovine seminal RNase pptx
Ngày tải lên :
16/03/2014, 23:20
... result also provides indirect evidence of correct pairing of the interchain disulfide bridges in the recombinant sample [11] The kinetics of the N-terminal arms swapping in the P19A-BSRNase is ... determinants for this process are located in regions different from the hinge loop 16–22 In the search for the region primarily involved in the swapping, we focused our attention on the interface ... (Fig 5B) Interestingly, the amount of the MxM form at the plateau as evaluated by integrating the peaks obtained on gel filtration is 70%, i.e in complete agreement with that obtained for the native...
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